On account of the disruption in education due to the corona pandemic, we're are providing a 7-day Free trial of our platform to teachers. Know More →

NEET

Explore popular questions from Biotechnology Principles and Applications for NEET. This collection covers Biotechnology Principles and Applications previous year NEET questions hand picked by experienced teachers.

Select Subject

Chemistry

Physics

Biology

Biotechnology Principles and Applications

Correct Marks 4

Incorrectly Marks -1

Q 1. Main objective of production/use of herbicide resistant GM crops is to

A

encourage eco-friendly herbicides

B

reduce herbicide accumulation in food articles for health safety

eliminate weeds from the field without the use of manual labour

D

eliminate weeds from the field without the use of herbicides.

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 2. A single strand of nucleic acid tagged with a radioactive molecule is called

A

vector

B

selectable marker

C

plasmid

probe

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 3. Restriction endonuclease

A

synthesizes DNA

B

cuts the DNA molecule randomly

cuts the DNA molecule at specific sites

D

restricts the synthesis of DNA inside the nucleus

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 4. In Lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain

minimal medium and streptomycin

B

complete medium and streptomycin

C

only minimal medium

D

only complete medium

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 5. Stirred-tank bioreactors have been designed for

A

addition of preservatives to the product

B

purification of the product

C

ensuring anaerobic conditions in the culture vessel

availability of oxygen throughout the process

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 6. Which part of the tobacco plant is infected by {tex}Meloidogyne \ incognita{/tex}?

A

Stem

Root

C

Flower

D

Leaf

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 7. The figure below shows three steps {tex} ( \mathrm { A } , \mathrm { B } , \mathrm { C } ) {/tex} of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents{tex}?{/tex}

A

{tex} \mathrm { B } {/tex} - denaturation at a temperature of about {tex} 98 ^ { \circ } \mathrm { C } {/tex} separating the two DNA strands

B

{tex} \mathrm { A } {/tex} - denaturation at a temperature of about {tex} 50 ^ { \circ } \mathrm { C } {/tex}

{tex} \mathrm { C } {/tex} - extension in the presence of heat stable DNA polymerase

D

{tex} \mathrm { A } {/tex} - annealing with two sets of primers

Explanation


Correct Marks 4

Incorrectly Marks -1

Q 8. How many of the Dipteran insects are killed by the proteins Produced from Bacillus thuringiensis?
[Beetles, Flies, Tobacco, Budworm, Armyworm]

A

None

One

C

Two

D

Three

Explanation

Dipteran insects specifically asked in the question are member of an order of insects containing the two-winged or so-called true flies. Beetles belong to order Coleoptera Tobacco plant belong to Polemoniales Budworm and armyworm belong to order Lepidoptara BT toxin produced by Bacillus thuringiensis are wide variety of insecticidal proteins active against certain insects like Dipterans, Coleopterans, and Lepidoptara. Therefore the answer for this question is 'One' "

Correct Marks 4

Incorrectly Marks -1

Q 9. Choose the great option regarding retrovirus

A

A DNA virus that can synthesise RNA during infection

An RNA virus that can synthesise DNA during infection

C

A ssDNA virus

D

A dsRNA virus

Explanation

 Retroviruses are a type of virus in the viral family called Retroviridae. They use RNA as their genetic material and are named for a special enzyme that’s a vital part of their life cycle — reverse transcriptase, that convert its RNA into DNA." 

Correct Marks 4

Incorrectly Marks -1

Q 10. The site of production of ADA in the body is

Lymphocytes

B

Erythrocytes

C

Blood plasma

D

Osteocytes

Explanation

 Lymphocytes are kind of white blood cells present in the bone marrow. ADA (adenosine deaminase) is an enzyme that is present in lymphocytes and is very important for the immune system to function. Its primary function in humans is the development and maintenance of the immune system." 

Correct Marks 4

Incorrectly Marks -1

Q 11. What is the true about Bt toxin

A

Bt protein exists as active toxin in the Bacillus

The inactive protoxin gets converted into active form due to acidic pH of the insect gut

C

The activated toxin enters the ovaries of the pest to sterilize it and thus prevent its multiplication

D

The concerned Bacillus has antitoxins

Correct Marks 4

Incorrectly Marks -1

Q 12. Bacillus thuringiensis forms protein crystals which contain insecticidal protein this protein.

A

Is activated by acidic pH of the gut of the insect pest

Binds with epithelial cells of midgut the insect pest ultimately killing it

C

Does not kill the carrier bacterium which is itself resistant to this toxin

D

All of these

Correct Marks 4

Incorrectly Marks -1

Q 13. GEAC stands for

A

Genome engineering action committee

B

Ground environment action committee

C

Genetic and environmental Approval committee

Genetic engineering approval committee

Correct Marks 4

Incorrectly Marks -1

Q 14. Bt cotton farming has shown good results in -

Malwa (Punjab)

B

Katihar (Bihar)

C

Kolkata (West Bengal)

D

Shimla

Correct Marks 4

Incorrectly Marks -1

Q 15. Several nematodes parasities -

A

A wide variety of plants

B

A wide variety of animals

C

Human

All

Correct Marks 4

Incorrectly Marks -1

Q 16. The first human drug made using recombinant DNA technology was -

A

Glyphosatase

B

TPA

Insulin

D

Erythropoietin

Correct Marks 4

Incorrectly Marks -1

Q 17. What is the disadvantage of using porcine insulin (from pig) in diabetic patients?

A

It leads hypercalcemia

B

It is expensive

It may cause allergic reactions

D

It can lead to mutation in human genome

Explanation

The insulin obtained from pancreas of pigs and cows were slightly different in structure i.e the 1-3 amino acids between the animal insulin and human insulin were different. When the animal insulin was administered by a diabetic patient he developed antibodies against the animal insulin thereby causing allergic reactions."

Correct Marks 4

Incorrectly Marks -1

Q 18. A doctor while operating on an HIV + patient accidentally cut himself with a scalpel. He comes to you, suspecting himself to have contracted the virus which test will you advise him to rule out / confirm his suspicion?

PCR

B

Routine urine examination

C

TLC

D

DLC

Explanation

 Very low count of bacteria or viruses (when the symptoms of the disease are not yet visible) can be detected by multiplication of their nucleic acid by PCR, (PCR can detect very low amounts of DNA). PCR is usually used to detect HIV in suspected AIDS patients."

Correct Marks 4

Incorrectly Marks -1

Q 19. In order for gene therapy to be most effective, genes should be inserted in -

A

WBC

B

RBC

Stem cells

D

All of the above

Correct Marks 4

Incorrectly Marks -1

Q 20. Transgenic animals are those which have -

A

Foreign DNA is some of its cells

Foreign DNA in all its cells

C

Foreign RNA in all its cells

D

Both a and c

Explanation

Transgenic animals have foreign DNA in all its cells. The animals which carry foreign genes are called transgenic animals. The foreign genes are inserted into the genome of animals using recombinant DNA technology or gene manipulation."

Correct Marks 4

Incorrectly Marks -1

Q 21. Transgenic rats, rabbits pigs, sheep cows and fish have produced although over 95 percent of all existing transgenic animals are -

A

Pigs

B

Fish

C

Rats

Mice

Explanation

Transgenic rats,rabbit,pigs,sheep,cows and fish have been produced, although 95 % of all existing transgenic animals are mice."

Correct Marks 4

Incorrectly Marks -1

Q 22. Pharming is a term that describes -

A

Animal used in transgenic research

B

Plants making genetically altered foods

C

Synthesis of recombinant drugs by bacteria

Synthesis of a protein drug in the milk of a transgenic animal

Correct Marks 4

Incorrectly Marks -1

Q 23. Which of the following statemnets is correct?

A

The current interest in the manipulation of microbes, plants and animal has raised serious ethical issues

B

One possible risk of genetic engineering is the accidental production dangerously resistant microorganisms

C

Although risks are possible, genetic engineering appears to offer more of a contribution of human welfare than threats

All

Correct Marks 4

Incorrectly Marks -1

Q 24. How many recombinant therapeutics have been approved for human-use the world over?

A

12

30

C

20

D

18

Correct Marks 4

Incorrectly Marks -1

Q 25. A transgenic food crop which may help in solving the problem of night blindness in developing countries is

Golden Rice

B

Bt Soyabean

C

Flavr Savr Tomato

D

Starlink Maize

Explanation

Rice is a staple food in many countries, particularly in Asia, but does not contain vitamin A or its immediate precursors. By inserting two genes from Daffodil and one gene from a bacterial species into rice plants, Swiss researchers have produce rice capable of synthesizing β-carotene, the precursor of vitamin A. Vitamin A is required by all individuals as it is present in retina of eyes. Deficiency of vitamin A causes night blindness and skin disorders. This rice is called 'Golden rice' because of yellow colour of rice grains due to the presence of β-carotene. So, the correct answer is 'Golden rice'."